Microcirculation and Vascular Regeneration Laboratory

Location: 38-134
Coordinator:  Dr. Trevor Cardinal

Spring Lab BBQ 2018
Spring Lab BBQ 2018

Principal Investigator
Trevor Cardinal, PhD

Graduate Students
Isobelle Espiritu
Ricardo Lasa
Jessica Scherer
Colleen Richards
Quentin Klueter

Undergraduate Students
Olivia Welch
Jordan Ambrose
Ada Tadeo
Nick Dion
Sammi Shi
Christine Do
Jaden Frazier
Elsa Bean
Karly Knox
Mahina Saucedo
Makenzie Jones
Randy Hau
Simon Park
Chloe Zimovets

Laboratory Mission Statement
The mission of our lab is to advance biomedical understanding while educating future generations of scientists and engineers, providing students with opportunities to learn by creating knowledge and ideas. Our primary objective is to understand the impact of vascular disease on blood flow control, which we hope will provide the foundation for advanced therapeutics to improve vascular health. 

General Research Interests
Our laboratory is at the nexus of two fields- microcirculation and cardiovascular regenerative medicine, hence the lab name. Specifically, we are interested in how the regulation of skeletal muscle blood flow is impacted by arteriogenesis* and risk factors for vascular disease. Studies investigating blood flow control are typical in the microcirculation field, while studies investigating arteriogenesis are typical in the cardiovascular regenerative medidine field. We combine these two fields to understand how various aspects of vascular disease impact the control of skeletal muscle blood flow. Unfortunately, vascular disease and its many risk factors impair the regulation of skeletal muscle blood flow, which can lead to leg pain while walking and decreased quality of life. Although all tissues depend on nutrient and waste exchange through blood, the massive increase in muscle metabolism that occurs when transitioning from rest to locomotion needs to be supported by a similarly massive increase in blood flow. When blood flow doesn’t increase sufficiently, patients experience a burning sensation referred to as intermittent claudication. If left untreated, vascular disease can worsen to the point where limb tissues no longer heal, and amputation is required (vascular disease is the #1 cause of lower limb amputation). Therefore, we hope to understand why various aspects of vascular disease impair the regulation of blood flow to skeletal muscle, and how cell transplantation and exercise can improve these processess blood flow. Although our focus is skeletal muscle, our research is applicable to vascular disease of the heart, aka coronary heart disease- the leading cause of death in the US.

*arteriogenesis is the enlargement of natural bypass arteries after a main artery is blocked

Laboratory Methods
Our studies involve one or more of the following- surgery, cell transplantation, in vivo microscopy, and immunofluroescence.

Publications (Lab Members Bolded)

Gouin KHHellstrom SK, Clegg LE, Cutts J, Mac Gabhann F, Cardinal TR, Arterialized collateral capillaries progress from non-reactive to capable of increasing perfusion in an ischemic tree, Microcirculation. 2017 Dec 28 [Epub ahead of print]

Guendel AM, Martin KS, Cutts J, Foley PL, Bailey AM, Mac Gabhann F, Cardinal TR, Peirce, SM, Murine Spinotrapezius Model to Assess the Impact of Arteriolar Ligation on Microvascular Function and Remodeling, Journal of Visualized Experiments, 73, 2013.

Cardinal TRStruthers KRKesler TJYocum MD, Kurjiaka DT, Hoying JB, Chronic hindlimb ischemia impairs functional vasodilation and vascular reactivity in mouse feed arteries, Frontiers in Physiology, 2:91, 2011

MCS Annual Meeting 2018- Cell Transplantation & Collateral Capillary Arteriogenesis, Cell Transplantation & Arterialized Collateral Capillary Vasodilation, Cell Transplantation & Arteriogenesis in Obesity, Force Production & Arterial Occlusion
MCS Annual Meeting 2017- Sex Differences in Arteriogenesis & Vasodilation
Fall MCS Meeting 2014- Blood Velocity & Collateral Capillary Arteriogenesis
MCS Annual Meeting 2014- Vasodilation & Collateral Capillary ArteriogenesisBlood Velocity & Collateral Capillary ArteriogenesisMechanoadaptation & Collateral EnlargementAortic Stenting
Fall MCS Meeting 2013- Vasodilation in Preexisting & Newly Formed Collaterals
MCS Annual Meeting 2013- Vasodilation & Collateral EnlargementVasodilation & Collateral Capillary Arteriogenesis
MCS Annual Meeting 2012- Ischemia & Bone
MCS Annual Meeting 2011- Vasodilation & Ischemia
MCS Annual Meeting 2010- Vasodilation & Ischemia
Fall MCS Meeting 2009- Vasodilation & IschemiaNetwork Architecure & Ischemia
MCS Annual Meeting 2009- Vasodilation & IschemiaNetwork Architecture & Ischemia

“Microcirculations” in Nature
After viewing photomicrographs of vascular networks so often, we have begun to “see” these elegant, almost artisitc structures everywhere. After exercising your left brain on the papers and posters above, exercise your right brain with the pictures below.

Gorgonian Coral photographed in the Great Barrier Reef by David Danzeiser looks a lot like the interconnected capillaries of the lung.

Roots from a Maple Tree penetrating a burl on a Large Western Red Cedar (according to Dr Matt Ritter) in Stanley Park, resemble microvessels feeding a solid tumor.

NameDegreeAfter Graduation
Mary Kate EvansBS, BME 2018PhD at U Penn
Christopher HatchBS, BME 2019PhD at UC Irvine
Mark LandonMS, BME-Regenerative Medicine 2019Scientist at ThermoFisher
Madison HubbardBMS, BME-Regenerative Medicine 2019Engineer at ViaCyte
Courtney Nickell R&D Engineer at Edwards Life Sci
Vahid HamzeinejadBMS, BME-Regenerative Medicine 2018, ReportEngineer at ViaCyte
Tanner Quon BMS, BME-Regenerative Medicine 2018, ReportPD Associate at Capricor
Chris TranBMS, BME-Regenerative Medicine 2018, ReportMD, Cal Northstate U
Eric MagillBMS, BME-Regenerative Medicine 2017, ReportSecond Lieutenant, US Marine Corps
Ethan TietzeBMS, BME-Regenerative Medicine 2017, Report, Senior ProjectResearch Associate, Leiber Institute 
Padon SivesindBMS, BME 2017, ThesisProcess Engineer, Kite Pharma
Britta NelsonBMS, BME 2017, Thesis, Senior ProjectR&D Engineer at Meditrina
Megan Chu BMS, BME 2016, ThesisSenior Project Manufacturing Engineer, NeoTract 
Kenny GouinBMS, BME-Stem Cell Research 2016, ReportSenior ProjectPhD, Cedars Sinai
Patrick PaineMS, BIO-Stem Cell Research 2016, ReportResearch Associate, Stanford
Laura BurckhardtBMS, BME 2015, ThesisSenior ProjectEP-TSS, St Jude Medical
Ashli SantosBS, BME 2015, Senior ProjectMedical Assistant Program
Travis SuttleMS, BIO-Stem Cell Research 2015, ReportR&D, Capcicor Therapeutics
Caitlin KoeroghlianBS, BME 2014, Senior ProjectBiomechanics Engineer, Biomechanica
Amanda KrallBS, BME 2014, Senior ProjectEP-TSS, St Jude Medical
Ashkon NehzatiBMS, BME 2014, Senior ProjectER Scribe, Apply to DO
Sara HellstromBMS, BME 2014, Senior ProjectThesisProcess Engineer, St Jude Medical
Jennifer Go

BMS, BME-Stem Cell Research 2014, ReportSenior Project

Project Engineer, NDC
Josh CuttsBMS, BME-Stem Cell Research 2014, ReportSenior Project PhD, Arizona State University
Paul HecklerBS, BMED 2014, Senior ProjectR&D Engineer, Altaviz
Stephan Papp

BS, BIO 2014

MD, University of Colorado
Allison Peck

BMS, BME 2013, Senior Project

Mech Engineer, Home Dialysis Plus
Robert ChaconMS, BIO-Stem Cell Research 2013, ReportLab Manager, Stanford/VA
Daniel HooverBS, BME 2013, Senior ProjectMD, Loma Linda University
Paige CzarneckiBS, BME 2012, Proprietary ProjectApplications Engineer, inviCRO
Ryan GallagherBMS, BME 2012, ThesisQuality Engineer, Alcon
Tyler SmithBS, BME 2012 Proprietary ProjectPhD, UCLA
Andrew TiltonBS, BIO 2012, Senior ProjectResearch Associate, SRI Int’l
Chris LiBS, BIO 2012MD, Boston University
Whitney Cole

BMS, BME-Stem Cell Research 2012, Report

ER Scribe, apply to MD
Ian MahaffeyBMS, BME-Stem Cell Research 2012, ReportQuality Engineer, Boston Scientific
David DanzeiserBMS, BME, 2012, ThesisWorld traveler
Alexander BynumBMS, BME 2011, ThesisQuality Engineer, Stryker
Michael GoveaBMS, BME 2011, ThesisR&D Engineer, Boston Scientific
Michael MachadoBMS, BME 2012, Senior ProjectThesis, Dr K Cardinal
Thomas HarperBMS, BME-Stem Cell Research 2011, ReportResearch Associate, UCSD
Anna McCannMS, BIO-Stem Cell Research 2011, ReportPhD, U of Washington
Andrew BurchMS, BIO-Stem Cell Research 2011, ReportPhD, UC Davis
Kyle StruthersBMS, BME 2011, ThesisEP-TSS, St Jude Medical
Shilpi GhoshMS, BME 2010, ThesisMBA, Indian Inst of Foreign Trade
Joe Zhu OD, New Engl College of Optometry
Phil Chang TDP, Edwards Life Sciences
Emily DeckertBS, BME 2010, Senior ProjectEP-TSS, St Jude Medical
Thomas KeslerBS, BME 2010, Senior ProjectMD, New York Medical College
Matthew YocumMS, ENGR (BME) 2009, ThesisMD, Medical College Wisonsin
Allyse AlexBS, ASCI 2008Clinical Embryology
Stephanie WoodMS, ENGR (BME) 2008, Thesis MD, UC Irvine